Potato Spindle Tuber Viroid-infected Tissues Contain RNA Complementary to the Entire Viroid
نویسندگان
چکیده
منابع مشابه
Genetic variability of potato spindle tuber viroid RNA replicon.
The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected fo...
متن کاملogenicity, viroid evolution, Citrus exocortis viroid, Potato spindle tuber viroid, Peach latent mosaic viroid, Chrysan- themum chlorotic mottle viroid
Viroids are autonomously replicating, small singlestranded circular RNA molecules that do not code for proteins and may cause disease in infected, susceptible plants. Viroids have the ability to induce both RNA-mediated transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS) in infected plants. Viroid PTGS has also been demonstrated in a wheat germ extract system. A p...
متن کاملPrecisely full length, circularizable, complementary RNA: an infectious form of potato spindle tuber viroid.
The replication of many viral and subviral pathogens as well as the amplification of certain cellular genes proceeds via a rolling circle mechanism. For potato spindle tuber (PSTVd) and related viroids, the possible role of a circular (-)strand RNA as a template for synthesis of (+)strand progeny is unclear. Infected plants appear to contain only multimeric linear (-)strand RNAs, and attempts t...
متن کاملPotato spindle tuber viroid: the simplicity paradox resolved?
TAXONOMY Potato spindle tuber viroid (PSTVd) is the type species of the genus Posipiviroid, family Pospiviroidae. An absence of hammerhead ribozymes and the presence of a 'central conserved region' distinguish PSTVd and related viroids from members of a second viroid family, the Avsunviroidae. PHYSICAL PROPERTIES Viroids are small, unencapsidated, circular, single-stranded RNA molecules which...
متن کاملViroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo
While biogenesis of viroid RNAs is well-known, how they decay is restricted to data involving host RNA silencing. Here we report an alternative degradation pathway operating on potato spindle tuber viroid (PSTVd), the type species of nuclear-replicating viroids (family Pospiviroidae). Northern-blot hybridizations with full- and partial-length probes revealed a set of PSTVd (+) subgenomic (sg)RN...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1982
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-59-1-139